Determination of 3 isothiazolinone preservatives in water-based adhesive by LC-MS/MS method

A method for determining 3 isothiazolinone preservatives in water-based adhesive by high performance liquid chromatographytandem mass spectrometry(LC-MS/MS) was developed.The sample was extracted with water,centrifugated,and then analyzed by LCMS/MS and quantified by internal standard method.Results showed that:the linear relationship was good with determination coefficients R2 ≥ 0.9996 in the MI and CMI concentration range of 2.5~250 ng/m L and in the BIT concentration range of 5~500 ng/m L.The recoveries of standard addition ranged from 96.3% to 109.6% with relative standard deviation(RSD) of 1.5%~6.7%.The limit of detection was between 0.011 and 0.015 mg/kg,and that of quantification was between 0.035 and 0.051 mg/kg.It was concluded that this method was suitable for the determination of 3 isothiazolinone preservatives in water-based adhesive.