Study on Establishment of Aseptic System of Malus halliana

Taking Malus halliana as test material,explant disinfection factors of Malus halliana and the best combination of culture medium and plant growth hormones concentration for bud germination were studied.The results indicated that the explants of best disinfectant effect were those which were young and sampled in April to August every year;the best disinfection method of stem segment with single bud was 75% alcohol 30 s with 0.1% HgCl2 6 min,the best disinfection method of leaf was 75% alcohol 30 s with 0.1% HgCl2 8 min,Because of high infection rate,it was too hard for Petal to establish aseptic system;Orthogonal experimental design showed that the best combination of culture medium and plant growth hormones concentration for bud germination was WPM+6-BA 1.0 mg/L+NAA 0.5 mg/L,and germination rate was 87%.