Über die Stimulierung des Aminosäure-Einbaus in vitro durch DNS in zellfreien Systemen aus Chlorella pyrenoidosa

The amino acid incorporation into protein by cell-free preparations of Chlorella pyrenoidosa is found to be stimulated by DNA isolated from various organisms. This stimulation is not affected by preincubation of the DNA with ribonuclease and by addition of ribonuclease to the reaction mixture. Streptomycin which reduces amino acid incorporation in the normal system stimulates slightly the incorporation in the presence of DNA. The incorporation rate is linearly dependent upon the amount of DNA in the reaction mixture from 10 to 100 µg DNA. Ribosomes from nitrogen-deficient cells exhibit a similar rate of stimulation by DNA. Deoxycholate-treated particles show much higher stimulation by added DNA than normal ribosomes do.