[Effect of Astragalus mongholicus injection on proliferation and apoptosis of hormone sensitive (MCF-7) breast cancer cell lines with physiological dose E2].

Objective:To investigate the effect of Astragalus mongholicus injection on proliferation and apoptosis in hormone sensitive breast cancer cell lines(MCF-7) with physiological dose E2. Methods:Cell control group、TAM control group and five different dose Astragalus mongholicus injection groups with physiological dose E2 were set in the study. Proliferation of MCF-7 cells were evaluated with MTT assay,cell apoptotic rate were measured with flow cytometry,DNA ladder and cell cycle were observed. Results:In physiological dose E2,Astragalus mongholicus injection inhibited MCF-7 cells proliferation at all concentration groups. As time lasting,Astragalus mongholicus injection showed better inhibitory effect than TAM(P0.05). Among 2×10-1g/mL～2×10-4 g/mL concentration,Astragalus mongholicus injection significantly increased the proliferative percent of G0/G1 and S-phase cell,decreased percent of G2-M phase cell(P0.05) at 24 hours. After cocultured 72 hours,Astragalus mongholicus injection increased the rate of apoptosis to 16.7% at 2×10-1g/mL concentration. Conclusion:In some dose limit, Astragalus mongholicus injection may inhibit proliferation,induce apoptosis and interrupt caryocinesia at G0-G1 phase or S phase in hormone sensitive (MCF-7) breast cancer cell lines with physiological dose E2.