Preparation of Antisera to the Reference Strains of Actinobacillus pleuropneumoniae and Serotype Identification of its Field Strains

Rabbits were inoculated with formaldehyde-inactivated bacteria suspensions of Actinobacillus pleuropneumoniae (APP)strains of 12 serotypes with bee glue as an adjuvant, to prepare a set of monospecific antisera against each serotypes of APP.Although there were some cross-reactions between the reference strains of different serotypes,any monospecific antiserum gave the highest titers to its homologous APP reference strain bacteria suspension in agglutination test,they reached titers of 6-8 log2 respectively.By use of the set of antisera,the field strains isolated from different areas in Shandong privince in 2003 were identified for their serotypes,they belong to serotypes 3(2 isolates)、4 (1 isolates)、5(4 isolates)and 7 (4 isolates), respectively.