Functional EGFP–dystrophin fusion proteins for gene therapy vector development

Plasmids, pCR3.1 mini-dystrophin and primers3500 males, is one of the most prevalent types of muscular Eukaryotic TA cloning kit containing the plasmid pCR3.1dystrophy and is characterized by the rapid progression of expression vector was purchased from Invitrogen (Carlsbad,muscle degeneration which occurs early in life. The mutated CA). EGFP cDNA was amplified by the polymerase chaingene in DMD, found on the X chromosome, normally encodes reaction (PCR) of the gene contained in plasmid pIRES-EGFPa large protein named dystrophin (427 kDa) which is required (Clontech, Palo Alto, CA) using the primers C and D showninside muscle cells for structural support (Acsadi et al.