Arrangement and method for light microscopy Journal

The invention relates to an arrangement for sheet light microscopy with an illumination device (3) for generating a light sheet (LB1) for illuminating a strip of a sample and with a detecting device (4) for detecting a light emitted by the sample the fluorescent radiation. The invention further relates to a corresponding method. Your task is to describe a device and method for light sheet microscopy, the recording speed can be increased without compromising the image quality or to increase the radiation exposure of the sample. This object is achieved by an illumination device which is arranged parallel for generating at least one of a first light sheet (LB1) other light sheet (LB2, LB3) for illuminating a further strip of the sample (1), and advantageously by a detection device (4) which is adapted for simultaneous detection of the arranged by the mutually parallel light sheets (LB1, LB2, LB3) excited fluorescence radiation. The object is further achieved by a method of the light sheet microscopy, in which a sample (1) with at least two mutually parallel light sheets (LB1, LB2, LB3) is illuminated, and in which for the detection of the fluorescence radiation of the illuminated by a light sheet strip of the sample the focal plane of this light sheet is made coincident with a detection plane of the light sheet.