[The molecular typing of clinical isolates Klebsiella pneumoniae producing beta-lactamases of extended specter of action.]

: The carbapenem resistant isolates of K. pneumoniae (4 strains) isolated from wound discharge of patients of burn department were analyzed with the purpose of molecular typing. The whole genome sequencing was implemented using highly productive sequenator MiSeq (Illumina, USA). Belonging of three isolates K. pneumoniae to sequence-type 395-serotype K2 is established. It is demonstrated that one isolate has a unique combination of sequence-type 23-serotype K57. The genetic determinants of main factors of pathogenicity and anti-microbial resistance are established and their localization is determined. All strains were characterized by availability of genes determining manifestation of invasive characteristics (fimbriae type I and III, proteinssiderofors), conditioning fast propagation in huma tissues. The genes responsible for synthesis of invasini and toxic substances such as α-hemolysin, enterohemolysin, shiga-like enterotoxins, cytotoxic necrotic factor, and hypermucoid phenotype regulator were not detected. In the structure of chromosome genes coding synthesis of β-lactamases of group SHV-1 and genes of resistance to quinolones and phosphomycin were detected. In the structure of mobilom a set of genes of resistance was detected including determinants of β-lactamases of larger specter of action CTX-M-15 and serin carbapenemases OXA-48 (ST395 isolates), in strain ST23 - only CTX-M-55. Previously, producers of CTX-M-55 of cefalosporinases among Russian isolates of Klebsiella pneumoniae were not detected. In all strains genes of β-lactamases OXA-1 and TEM-1, genes of resistance to aminoglycoside, fluoroquinolone, phoenicols, sulfonamides and trimethoprim are present. Additionally, in one isolate were detected genes determining resistance to macrolid. The obtained results provide supplementary information about molecular genetic characteristic of carbapenem resistant strains of Klebsiella pneumoniae circulating in the Russian Federation.