Short Communication: No Evidence of Occult SHIV Infection as Demonstrated by CD8+ Cell Depletion after Chemoprophylaxis-Induced Protection from Mucosal Infection in Rhesus Macaques

ABSTRACT Preexposure prophylaxis (PrEP) with antiretroviral drugs constitutes a promising strategy for HIV prevention. Potent PrEP regimens with reverse transcriptase inhibitors can prevent detectable SHIV infection in a repeated low-dose macaque model that resembles human transmission, supporting plans to quickly move this approach into human trials. However, the possibility remains that extremely low levels of virus replication could nonetheless occur during PrEP and seed viral reservoirs in tissues. Therefore, seemingly protected macaques may harbor occult virus that may be initially contained by cytotoxic T cells, but could emerge later. To explore this possibility, we studied whether CD8+ cells suppress viremia in four rhesus macaques apparently protected by daily or intermittent Truvada (FTC and tenofovir) during 14 low-dose, rectal SHIVSF162P3 challenges and during a subsequent drug washout period. CD8+ cells were efficiently ablated with antibodies in these and two additional control macaques that...