Xanthine-Xanthine Oxidase系におけるSuperoxide産生に及ぼす各種抗アレルギー薬の影響

Effects of six antiallergic drugs on superoxide (O2-) production in xanthine-xanthine oxidase system were studied by the method of ESR spin trapping and measuring absorbance at 550 nm of ferricytochrome C.5 5-Dimethy1-1-pyrroline-N-oxide (DMPO) was used as a spin trapping agent.At the drug concentration of 600μM, only two drugs, tranilast (N-5') and amlexanox (AA-673), decreased significantly the signal intensity of DMPO-OOH adduct and the initial change in absorbance at 550nm per minute, indicating the O2- scavenging effect of these drugs.The O2- scavenging effect of AA-673 was stronger than that of N-5'. At the concentration of 30μM, it was recognized that AA-673 decreased O2-production more than 60% but not N-5'. These results indicate that AA-673 has the superoxide dismutase-like activity.