DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

ABSTRACT Vitamin B 12 is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B 12 deficiency have an underlying stomach or intestinal disorder that limits the absorption of vitamin B 12 and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B 12 was detected by using ELISA. The vitamin B 12 concentrations of milk samples obtained from the cows of Holstein, Jersey, Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B 12 in Zavot cows milk were higher than the other species. Key words: Vitamin B 12 ,  cow milk, ELISA. FARKLI SIĞIR IRKLARINA AIT SUT ORNEKLERINDE B 12 VITAMIN DUZEYININ ELISA TEKNIĞI ILE TESPITI OZET B 12 vitamini, B kompleks vitaminlerin bir uyesidir. Bu vitamin kobalt icerdiginden dolayi kobalamin olarak adlandirilmaktadir. B 12 vitamini balik, et, tavuk, yumurta ve sut urunlerinde bulunur ve bakteriler tarafindan sentezlenir. B 12 vitamini yetersizligine bagli olarak mide ve bagirsak bozukluklar, kol ve bacaklarda uyusukluk ve megolablastik anemi sekillenir. Bu calismada farkli sigir irklarina ait sut orneklerinde B 12 vitamin duzeyleri arastirildi. Bu amacla Samsun ve Kars bolgesinden Holstayn, Jersey, Zavot and Dogu Anadolu Kirmizisi sigir irklarina ait sut ornekleri toplandi. Sut orneklerinde B 12 vitamini miktari ELISA yontemi ile tespit edildi. B 12 vitamini Holstayn, Jersey, Zavot and Dogu Anadolu Kirmizisi sigir irklarina ait sut orneklerinde sirasiyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapilan istatistik calismasi sonucunda vitamin B 12 miktari Zavot irkina ait sut orneklerinde diger irklardan daha yuksek bulundu (p<0.05, ANOVA). Anahtar Kelimeler: B 12 vitamini, inek sutu, ELISA.