Interferon-γ-induced regulation of the pancreatic derived cytokine FAM3B in islets and insulin-secreting βTC3 cells

Abstract The pancreatic-derived factor (PANDER, FAM3B) is a novel protein that is β-cell specific and induces β-cell death. PANDER is localized to insulin-containing granules-based on confocal microscopy and immunogold electron microscopy. PANDER protein was detected in the conditioned medium of βTC3 cells. Using real-time reverse transcription-polymerase chain reaction, treatment of βTC3 cells with IL-1β + TNFα + IFNγ induced a significant seven-fold increase in PANDER mRNA expression ( n  = 3; p p n  = 3; p