Somatic embryogenesis from stem explants of Aesculus hippocastanum

Somatic embryogenesis was obtained when stem explants of Aesculus hippocastanum L. were cultured in vitro on agar-solidified MS medium supplemented with 9.3 μM kinetin, 10.7 μM 1-naphthaleneacetic acid and 9.0 μM 2,4-dichlorophenoxyacetic acid. Plantlets were obtained in growth regulator-free medium.