[Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa].

Objective: To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa,in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R.glutinosa.Method: Leaves from test-tube seedlings of 85-5 R.glutinosa were used as explants.After rooting induction,they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R.glutinosa.Result and Conclusion: NAA played a significant role in induction of test-tube tuberous roots of R.glutinosa,followed by sucrose and 6-BA.With leaves from test-tube seedlings as the explants,the optimal medium for inducing test-tube tuberous roots of R.glutinosa was MS+BA 3.0 mg·L-1+NAA 0.1 mg·L-1+sucrose 7%.The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R.glutinosa.