HPLC determination of 5,7-dihydroxychromone and eriodictyol of peanut hulls

A HPLC method was established for determination of 5,7-dihydroxychromone and eriodictyol of peanut hulls.The methods used a Kromasil C18 Column(4.6mm×150mm×5μm)as stationary phase and a mixture of methanol∶1% phosphoric acid(48∶52,v/v)as the mobile phase.The detection wavelength was 254nm and the flow rate was 1mL/min.The result showed the standard curve was linear(r=0.9999)in the range of 0.064～0.48μg of 5,7-dihydroxychromone and also linear(r=0.9999)in the range of 0.080～0.60μg of eriodictyol.The pretreatment method is simple,rapid and the results were accurate,so,the method is valuable for the controlling quality for peanut hulls and its products.