The Antidiabetic and Anti-microbial Activity Analyses in The Extract of Methanol, Ethyl Acetate, and Hexane from Flesh of Matoa (Pometia pinnata J. R. Forst. & G. Forst)

The current research was designed to examine the antidiabetic and anti-microbial activities in the extracts of methanol, ethyl acetate and hexane from Flesh of Matoa (Pometia pinnata J. R. Forst. & G. Forst) to find out the best solvent to produce antidiabetic and anti-microbial active compounds.  In the phytochemical screening, hexane extracted alkaloids and terpenoids, while ethyl acetate extracted saponin and methanol extracted tannin, phenol and terpenoids. In the antidiabetic activity by a-glucosidase inhibition methods, ethyl acetate showed the highest percentage of inhibition and the smallest value of IC50, followed by hexane and methanol compared to the Quercetin as the control group. The maximum percentage inhibition was 36.74 ± 0.07 for 100 µg/mL of concentration. The minimum value of IC50 was observed at 159.74 ± 0.65 µg/ml compared to the control group 6.04 ± 0.14 µg/ml. The smaller the IC50 value, the stronger the antidiabetic activity. Anti-microbial inhibition activities assessed using anti-microbial diffusion test method. The hexane and ethyl acetate extracts provide an inhibition of fungi and bacteria compared to Ketoconazole and Amoxicillin as the standard. The maximum fungi inhibition zone was observed at 4 mm for A. flavus and 9 mm for A. niger by Hexane extracts compared to the standard 14 and 24 mm. The maximum bacteria inhibition zone was observed at 7 mm for B. cereus by both of hexane and ethyl acetate extracts compared to the standard 5 mm. Compared to the anti-microbe inhibitory power categories, hexane and ethyl acetate was on the range of weak and moderate. The methanol extract could not inhibit all types of microbe. This study concluded that various extract of flesh of Matoa showed antidiabetic and anti-microbial activities at different value with varying potencies. The ethyl acetate extract was found to be more potent among all, followed by hexane and methanol. HIGHLIGHTS Phytochemical screening of Flesh of Matoa (Pometia pinnata R. Forst. & G. Forst) produced alkaloids, terpenoids, saponin, tannin, phenol and terpenoids In the antidiabetic activity by a-glucosidase inhibition methods, ethyl acetate showed the highest percentage of inhibition and the smallest value of IC50, followed by hexane and methanol The hexane and ethyl acetate extracts provide an inhibition of fungi and bacteria compared to Ketoconazole and Amoxicillin as the standard GRAPHICAL ABSTRACT