A Survey Using Normal Phase Hplc of Aflatoxins in Domestic and Imported Foods and Dairy Products in the Ussr

AbstractAn improved normal phase HPLC method has been developed to study the occurrence of aflatoxins B1, B2, G1, G2 and M1 in domestic and imported foods. Ether-methanol-water (95:5:1) mobile phase and fluorometric detector with silica gel packed flow cell were used. The detection limit of the method was 0.1 ug/kg for aflatoxin B1, coefficients of variation were 11% and 8.5% at contamination levels 10 and 100 ug/kg of aflatoxin B1 respectively. Recoveries of added aflatoxins B1, B2, G1, G2 for corn ranged from 78% to 88%. This method allows the determination of aflatoxins B1, B2, G1, G2, and M1, B2a, M2a as well as other aflatoxin metabolites. The method is used in monitoring aflatoxin contamination of foods, the first stage of which is a preliminary screening of samples by TLC method (the detection limit is 1.0 ug/kg for aflatoxin B1). A survey of the occurrence of aflatoxins B1, B2, G1, G2, in Soviet domestic and imported cereals, nuts, beans, and oil seeds harvested in 1985-87 (over 4300 samples) as w...