The study of regeneration mode of end-to-side neurorraphy

Abstrat Objective To study the regeneration mode of end-to-side neurorraphy by fluroneurotrace and histological method. Methods 24, female, SD rats were divided into 3 groups: normal group, end-to-end group, end-to-side group. 3 months after models were maded, 2 rats were sacrificed in every group , 0.5cm length of coaptation area were achieved and made slices longititudely, then were dyed by HE and examed histologically . The other rats were used for retrograde neurotrace. Tibia nerves were in 0.2% Diamidino Yellow and perosneal nerve were in 0.2% True Blue.7～10 days later, L4-L6 DRGs and correspond spinal cord were achieved. 40μm-thinkness slices were observed under the fluromicroscope. Results Regenerated axons of end-to-side neurorraphy group were found from tibia nerves to personeal nerves by histological examination. And the neuroiums of tibia nerve had its continuous. Retrograde neurotrace showed that double labeled cells with TB and DY in L4-L6 DRGs (mainly were L4,L5) and in lateral of anterior corner in correspond spinal cord were only found in end-to-side group. Most of double cells in DRG were middle and small cells. And most of double cells in spinal were larger than 50μm.There were no double label cells in end-to-end group and normal group. Conclusion The mode of nerve regeneration in end-to-side neurorrsphy was collateral regeneration. And both motor and sensor fibers were regenerated. Majority of sensor fibers were related to sensation of pain.