Cloning and sequence analysis of P81 gene of eight strains Myciokasna bovis in China.

Eight isolates of Myciokasna bovis were isolated from lung tissues afected cattle with respiratory disease from eight provinces. According to the reported nucleotide sequence of P81 gene of the Myciokasna bovis PG45 stain,a pair of primers was designed for amplification of the P81 gene. Then the amplified fragments were cloned into pMD-18T vector and sequenced. The results showed that the P81 gene was 2 097 bp in length,and included a complete open reading frame encoded a protein of 699 amino acids. The homology of nucleotide sequences for these eight isolates were 99.4 % to 99.9 % in P81 gene,94.6 % to 94.9 % with PG45 strain,and 73.8 % to 74% with M. agalacia PG2 strain. These results indicted that P81 gene of M. bovis were highly conserved,but difference between species,and the P81 gene is potential gene to be used in vaccine and diagnosis.