DNA Fingerprinting for Identification of Rice Varieties and Seed Genetic Purity Assessment

Maintenance of seed purity is an important skill-demanding aspect in seed production and multiplication. DNA fingerprinting allows identification of plant genotypes with high precision and stability as it is not influenced by environment, epistatic interactions and pleiotropic effects. DNA barcodes, the unique pattern of SSR polymorphism from the allelic variation data were developed for 14 visually similar varieties of medium grained rice and eight varieties of long slender grain types, having high market demand. Single-tube multiplex assays and DNA barcodes were generated using the available data on 32 markers for medium slender and 35 markers for long slender varieties to make variety identification easy and precise, which can supplement traditional standard practices in determining purity and certification.