Erythrocyte 2,3-diphosphoglycerate in anaemic sheep

Experimental anaemia resulted in an increase of red cell 2,3-DPG from 0.11 μM/gHb to 0.99 μM in haemoglobin A sheep and from 0.21 μM to 1.5 μM in haemoglobin B sheep. Production of haemoglobin C as a result of anaemia was confined to haemoglobin A only. The results, therefore, appear to suggest that the rise in 2,3-DPG in the red blood cells of different haemoglobin types is independent of haemoglobin C.