Characterization of short incubation time effects on chromogen signal obtained by HER2-expressing breast carcinomas using microfluidic immunohistochemistry

We present a microfluidic tissue processor (MTP) for detection of the human epidermal growth-factor receptor 2 (HER2) with immunohistochemistry (IHC) on clinical breast cancer tissue sections using chromogenic detection. In particular, we investigate the effect of short incubation times in resultant chromogenic signal for an accurate quantification.