Kit for quantitative detection of specific antibodies of treponema pallidum and preparation method thereof

The invention discloses a kit for quantitative detection of specific antibodies of treponema pallidum and a preparation method thereof and relates to the treponema pallidum. The kit is provided with an external packaging box, an alkaline-phosphatase-labeled monoclonal antibody bottle, a luminous substrate bottle, 6 types of calibrator bottles containing the specific antibodies of the treponema pallidum with different concentrations, a washing liquid bottle and a recombinant-antigen-coated microporous plate, wherein the alkaline-phosphatase-labeled monoclonal antibody bottle, the luminous substrate bottle, the 6 types of calibrator bottles containing the specific antibodies of the treponema pallidum with different concentrations, the washing liquid bottle and the recombinant-antigen-coated microporous plate are arranged in the external packaging box. The preparation method comprises the following steps: preparing specific recombinant antigens of the treponema pallidum, the recombinant-antigen-coated microporous plate, natural membrane protein monoclonal antibodies, alkaline phosphatase labels of the monoclonal antibodies, a luminous substrate, a washing liquid and calibrators successively, and finally assembling into the kit for quantitative detection of the specific antibodies of the treponema pallidum. The kit can be used for quantitative detection of the specificity of the syphilis in clinical specimens and can be used as an objective index of the treating effect and the prognosis of the syphilis.