Anxiolytic and anticonvulsant effects of dioclenol flavonoid isolated from stem bark of dioclea grandiflora on mice

Summary: The aim of the present study is to demonstrate the anxiolitic and anticonvulsant effect of fraction (alcoholic) obtained from the stem bark of Dioclea grandiflora (Dg) and Dioclenol on mice using several behavioural assays. Groups of mice treated by the intraperitoneal (i.p.) route with doses of 15, 30, and 60 mg/kg (i.p.) of the fraction and Dioclenol with a dose of 10 mg / kg showed significant action in the Elevated Plus-maze (EPM) (time spent in open arms and time in spent in the closed arms). The Hole-board Test also showed a significant increase in the time spent in the Head-dip and Marble-Burying Tests. The same treatment increased the duration of the sleeping time induced by Sodium Pentobarbital, and showed a significant increase in protection against Pentylenotetrazole induced convulsion. These results indicate an anxiolitic-like and anticonvulsant-like effect of the fraction of stem bark of Dg and Dioclenol in mice . The phytochemical analysis suggests that the alcoholic fraction has higher concentration of flavonoid active (Dioclenol) and deserves further analysis. The studies conducted with the Dioclea grandiflora , can contribute, in the long term, in the field of its action in the CNS this flavonoid. Industrial relevance: The studies conducted with the Dioclea grandiflora , can contribute, in the long term, in the field of its action in the CNS.