Taishania pollutisoli gen. nov., sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil

Strain CZZ-1T was isolated from long-term TBBPA-contaminated soil Zaozhuang city, Shandong province, People’s Republic of China. CZZ-1T was pink-pigmented, Gram-stain-negative, rod-shaped, non-motile and aerobic. The 16S rRNA gene analysis indicated that strain CZZ-1T shows high similarities to Fluviicola taffensis DSM 16823T (92.6%) and Fluviicola hefeinensis KACC 16597T (92.5%) and less than 91% sequence similarities to other genus or species in the family Crocinitomicaceae. It was able to grow at 10–37 °C, with 0–6% (w/v) NaCl. It could hydrolyze gelatin, but could not reduce nitrates to nitrites. The predominant fatty acids of strain CZZ-1T were iso-C15:0 (51.3%), C15:0 2-OH (11.0%), iso-C17:0 3-OH (8.0%), C14: 0 (7.0%), iso-C15:1 G (6.8%) and Summed Feature 3 (C16:1 ω7c and/or C16:1 ω6c, 4.4%). The polar lipid profile was composed of five unidentified lipids, two unidentified phospholipids, one phosphatidylethanolamine, one unidentified aminolipid and one unidentified glycolipid. The predominant respiratory quinone was MK-6. The genomic DNA G+C content of strain CZZ-1T was 41.5 mol%. Based on data from phenotypic, chemotaxonomic and genotypic analysis in this study, strain CZZ-1T represents a novel species in a new genus in the family Crocinitomicaceae, for which the name Taishania pollutisoli gen. nov., sp. nov. is proposed. The type strain is CZZ-1T (= KCTC 52343T = GDMCC 1.2270T).