Twenty-Four–Hour Ex Vivo Perfusion with Acellular Solution Enables Successful Replantation of Porcine Forelimbs

Background:A critical barrier to successful limb replantation and allotransplantation is the maximum allowable limb ischemia time of 4 to 6 hours. The current gold standard is to preserve amputated limbs on an ice slurry. Experimental machine perfusion has yielded promising results as an alternative