[Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells].
2017
OBJECTIVE: To investigate the effects of oxygen-glucose deprivation (OGD) on the functions of blood-brain barrier in vitro, we established an OGD model to mimic cerebral ischemic injury in human umbilical vein endothelial cells (HUVECs). METHODS: Cells were cul-tured in DMEM without glucose under hypoxic conditions (0.3% O2) for different time point. The survival rate of HUVECs was detected by CCK-8 assay. The permeability of HUVECs was measured by epithelial volt-ohm meter. The expression of tight junction protein was detected by Western blot analysis. RESULTS: In OGD group, the survival rate of HUVECs was obviously decreased. The permeability of HUVECs also decreased in OGD-treated cells. And the expression of tight junction protein was reduced after OGD treatment. CONCLUSIONS: Our data indicate that OGD treatment disrupt the barrier function of tight junctions and increase permeability of HUVECs and finally accelerated cell death.
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