Comparative study on curcumin of Curcuma species from Nanpanjiang and Beipanjiang District, Guizhou Province.

[Objective] To study the content of curcumin in Curcuma species sampled from Nanpanjiang and Beipanjiang District,Guizhou Province,so as to provide scientific basis for quality evaluation of related medicinal materials.[Method] The content of curcumin,demethoxycurcumin and bisdemethoxycurcumin in the root and/or rhizome of six Curcuma species was detected by HPLC.HPLC analysis was performed on Inertsil ODS-3 column with detection wavelength at 420 nm.The mobile phase consisted of anhydrous methanol,isopropyl alcohol,water and acetic acid(V/V/V/V,20∶ 27∶ 48∶ 5),and the flow rate were 0.5 ml/min.[Result] The content of curcumin came from Anlong species was higher than others 5.94%,and the one came from Xingyi and Zhenfeng was lowest.Different original plants of RADIX CURCUMAE sampled from Xingyi varied greatly in the content of curcumin.Of these,the content of curcumin was the highest in RHIZOMA CURCUMAE LONGAE(3.27%),followed by RADIX CURCUMAE(0.005 4%) and RHIZOMA CURCUMAE(0.001 5%).[Conclusion] This method is simple and fast.The experimental result can provide scientific reference for quality evaluation of Curcuma medicinal materials from Nanpanjiang and Beipanjiang District,Guizhou Province.