Metabolic profiling of dairy cows affected with subclinical and clinical mastitis

The study was conducted to determine the effect of subclinical mastitis (SCM) and clinical mastitis (CM) on biochemical parameters of cows. A total of 200 samples of milk and blood, were collected from different areas of District Rajouri of J&K state. Before collection of blood and milk samples, the selection of animals from which samples had to be taken were put forward to screening by different cowside tests i.e. California Mastitis Test, electrical conductivity and pH. Subclinical mastitis were screened by California Mastitis Test (CMT), milk pH and electrical conductivity (EC) of milk. After screening Blood and milk samples were collected from 120 healthy, 50 SCM and 30 CM affected cows. Blood samples were analyzed for biochemical parameters. The present study was designed to study the possible biochemical profile (Glucose, Triglyceride, Albumin, Cholesterol, Globulin, HDL and TP) in the plasma of selected animals. Our study showed that total protein concentration was significantly (p≤0.05) higher in subclinical and clinical mastitic animals, compared to the control groups. Albumin level of the subclinical and clinical infected animals was significantly (P< 0.05) lower than the control group. Other parameters like glucose, cholesterol, triglyceride and HDL of subclinical and clinical mastitic animals showed non significant results as compared to the control group. Further, significant (p≤0.05) increase was observed in EC and pH of milk from SCM and CM infected animals compared to the control groups. Our study demonstrates that there is a significant increase in the levels of total protein, EC and pH in mastitic animals as compared to control groups and a significant reduction in the levels of albumin with the mastitic groups compared to control groups. Also glucose, cholesterol, triglyceride and HDL of subclinical and clinical mastitic animals showed non significant results as compared to the control group.