HPLC analysis of E. coli rRNA on ASAHIPAK GS-520 and nucleobases, nucleosides and nucleotides on ASAHIPAK GS-320.

: High-performance gel filtration chromatography using packed column Asahipak GS-520 was employed to separate E. coli 23S, 16S and 5S rRNAs. Low-molecular-weight components of nucleic acid were separated with Asahipak GS-320. Concurrent, rapid analysis of nucleobases, nucleosides and nucleotides was obtained isocratically. The elution of these substances is also described.