Method for preparing high-purity paclitaxel from taxus chinensis cell culture fluid

The invention relates to a method for preparing paclitaxel, and in particular relates to a method for preparing high-purity paclitaxel from taxus chinensis cell culture fluid. The method comprises the following steps of filtering the cell culture fluid, performing adsorptive enrichment to obtain a crude extract by using macroporous resin, performing normal-pressure alkaline alumina column chromatography and C18 reversed-phase preparative high-performance liquid separation, collecting components stepwise, performing centrifugation to obtain precipitates, and freeze-drying the precipitates to obtain high-purity paclitaxel monomers. According to the method, the high-paclitaxel yield cell culture fluid is used as a raw material; compared with extracts of taxus chinensis branches, leaves, barks and the like, the cell culture fluid contains few impurities such as pigments and lipid; compared with fungal fermentation liquid and bacterial fermentation liquid, the cell culture fluid contains more paclitaxel, and is convenient to extract; in addition, the paclitaxel in the taxus chinensis cell culture fluid is enriched directly by using the macroporous resin, so that the use of a great amount of organic solvent is avoided; a process is simple, economical, energy-friendly and high in recovery rate, and the obtained paclitaxel is high in purity.