VALUES OF SPERM THIOBARBITURIC ACID- REACTIVE SUBSTANCE IN FERTILE MEN
2002
Abstract Background : The objective of this study was to establish for the first time reference levels of sperm malondialdehyde, a stable lipid peroxidation product, in a cohort of fertile men. Methods : Sperm malondialdehyde, a thiobarbituric acid-reactive substance, was assayed using the 2-thiobarbituric acid method. Results : Sperm malondialdehyde levels, expressed in nM/10 8 spermatozoa, were normally distributed in our cohort of fertile men and averaged 0.0287±0.0162 (mean±S.D.). Conclusions : Given the impact of lipid peroxidation on spermatozoa and thereby on male fertility, the assay of sperm membrane thiobarbituric acid-reactive substance is clearly of interest. Malondialdehyde levels found in our study form a basis for normal values of sperm thiobarbituric acid-reactive substance observed in the semen of fertile men.
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