Preliminary Study on Gene Expression Differences between Normal Leaves and Albino Leaves of Anji Baicha (Camellia sinensis cv. Baiye1)

Differences of gene expression between normal and albino leaves of tea plant(Camellia sinensis cv.Baiye1) were studied by mRNA differential display reverse transcription-PCR(DDRT-PCR).Fifty-eight differentially expressed cDNA fragments were obtained from normal and albino leaves.Twelve of which were confirmed by the analysis of RT-PCR.Among them,5 fragments were specially expressed in normal leaves,4 fragments were specially expressed in albino leaves,1 fragment was up-regulated in normal leaves,and 2 fragments were up-regulated in albino leaves.Through BLASTX analysis with GenBank,5 cDNA fragments showed high homologous to five known genes,namely soul heme-binding family protein of Arabidopsis thaliana,methionine synthase of Beta vulgaris,gag-pol polyprotein-related in Medicago truncatula,human glyceraldehyde-3-phosphate dehydrogenase pseudogene and ACC synthase of rose,respectively.The other 7 might be novel genes,they showed poor homologous to all proteins in the database.