Isolation of cardiac ventricular myocytes from newborn rats by use of fractional elutriation centrifugation

Abstract Isolation of cardiac ventricular myocytes from newborn rats (0–4 days old) by use of elutriation centrifugation is described. By the use of fractional centrifugation, a homogeneous myocyte population with high purity was obtained without any further procedures, yielding about 10 6 myocytes/rat. When 48-hr monolayer cultures were established, the cells showed normal pulsatory contractions. A morphological evaluation of such cultures is given.