Bioactive extract of Fomitopsis pinicola rich in 11-α- acetoxykhivorin mediates anticancer activity by cytotoxicity, induction of apoptosis, inhibition of tumor growth, angiogenesis and cell cycle progression

Abstract Fomitopsis pinicola (Sw.:Fr.) P. Karst is used in Chinese and Korean traditional medicine The study was aimed to evaluate the anticancer activity of bioactive extract of F. pinicola, mechanism of activity and to identify the bioactive molecules. Ethyl acetate extract (EAE) of F. pinicola was assayed for cytotoxicity, antitumor activity, inhibition of cancerous skin papilloma, antiangiogenesis, inhibition of cell cycle progression and induction of apoptosis. Results showed significant cytotoxicity (IC50 of 100 µg/mL), inhibited tumor growth at a dose of 500 mg/kg, angiogenesis and cell cycle progression at G1 phase. HPTLC analysis of EAE showed 13 peaks indicating the chemical profile. LC-MS analysis revealed 11-α- acetoxykhivorin (RT 9.966, C14H44O12, MW 644.7095) as the major active chemical component. The results concluded that EAE of F. pinicola possessed significant antineoplastic activity. The observed anticancer activity might be assigned to the major chemical components of the extract.