Lily mottle virus double-antibody sandwich enzyme-linked immunosorbent assay kit and preparation method

The invention relates to a lily mottle virus (LMoV) double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) kit and a preparation method. The assay kit comprises a 96 micropore ELISA plate, an enzyme labeled antibody, a buffer solution, a developing solution and a stop solution, wherein the 96 micropore ELISA plate is coated with an LMoV specific polyclonal antibody, and the enzyme labeled antibody is an LMoV specific polyclonal antibody labeled by horse radish peroxidase. An immunogen used in the invention is a fusion recombinant antigen of LMoV coat protein and cytoplasmic inclusion protein, which is expressed by genetic engineering. The double-antibody sandwich enzyme-linked immunosorbent assay established by antibodies produced by the immunization can be used for detecting lily leaf specimens. Compared with other detection methods, the double-antibody sandwich enzyme-linked immunosorbent assay has high detection sensitivity and high accuracy, and ng-level viral antigenscan be detected. The preparation method of the kit is simple, convenient, quick, economical and practical.