Spatial Distribution and Preservation of Carbon Isotope Biosignatures in Freshwater Microbialite Carbonate

Understanding formation mechanisms of modern microbialites enables interpretation of biosignatures associated with fossilized stromatolites. Photosynthetic influences on carbonate precipitation are one proposed mechanism. Photosynthetic isotope biosignatures (13C) associated with freshwater microbialites in Pavilion Lake, British Columbia were widespread through the lake but less prevalent with increasing depth. Importantly, they were variably detectable on the exterior surface of individual microbialites. At depths ≤18 m, microbialite surface carbonates, associated with either nodular microbial communities or surface biofilms, had δ13Ccarb values up to +3.7‰ that were 13C-enriched above the predicted range of δ13Ccarb values for equilibrium precipitation from bulk ambient lake water dissolved inorganic carbon (DIC) (predicted mean δ13Ccarb = −0.2 ± 1.3‰). Vertical profiles of exterior, non-nodular biofilm present on microbialites collected from depths of 21 m and below showed instances of 13C-enrichment ...