Scalable Downstream Strategies for Purification of Recombinant Adeno- Associated Virus Vectors in Light of the Properties
2015
Recombinant adeno-associated virus (rAAV) vector is one of the promising delivery tools
for gene therapy. Currently, hundreds of clinical trials are performed but the major barrier for clinical
application is the absence of any ideal large scale production technique to obtain sufficient and highly
pure rAAV vector. The large scale production technique includes upstream and downstream processing.
The upstream processing is a vector package step and the downstream processing is a vector purification
step. For large scale downstream processing, the scientists need to recover rAAV from dozens of liters of cell
lysate or medium, and a variety of purification strategies have been developed but not comprehensively compared till
now. Consequently, this review will evaluate the scalable downstream purification strategies systematically, especially
those based on the physicochemical properties of AAV virus, and attempt to find better scalable downstream strategies for
rAAV vectors.
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