Protein Engineering mit nicht-kanonischen Aminosäuren
2012
Non-canonical amino acids as building blocks for protein biosynthesis facilitate modifications that go far beyond classical genetic engineering. Two complementary techniques allow either site-specific or residue-specific incorporation. Accordingly, the possible modifications range from atomic changes to the alteration of the overall biophysical characteristics of a protein. In this article, we present the state of the art of this new protein engineering approach.
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