Effects on the Content of the Combined Anthraquinone in the Minor Purgative Decoction by Different Decocting Time

Objective:To investigate the influence of different decocting time on the content of combined anthraquinone in the Minor Purgative Decoction,in order to determine the best decocting time of the rhubarb.Methods:The contents of the combined anthraquinone in the Minor Purgative Decoction of different decocting time were determined by HPLC.Waters XBridge C18(4.6mm×250mm,5μm) Column was applied.The mobile phase was a mixed liquid with MeOH-0.1% H3PO4(85∶15).The flow rate was 1 ml/min.The detection wavelength was 254 nm for anthraquinon and the column temperature was set up at 25 ℃.Results:The content of combined anthraquinone in the Minor Purgative Decoction at the highest levels when the rhubarb's decocting time was 30 minutes.Conclusion: The best decocting time of the rhubarb in the Minor Purgative Decoction is 30 minutes.