SPECTROPHOTOMETRIC ESTIMATION OF CHLORTHALIDONE IN PHARMACEUTICAL FORMULATION USING MBTH & FC REAGENT

Two visible spectrophotometric methods have been developed for the determination of Chlorthalidone either in pure form or in their pharmaceutical formulations. The developed methods are based on reaction of Chlorthalidone with MBTH, FC Reagent. They are quantified spectrophotometrically at their absorption maximum at 540nm (Method A), 680nm (Method B). Beer’s law was obeyed in the concentration ranges of 10-50 µg/ml, 10-50 µg/ml and 10-50 µg/ml for the two methods respectively. The colors were found to be stable for more than 4 hours. The proposed methods were successfully applied for determination of the Chlorthalidone in their pharmaceutical formulations and the results compared favorably to that of reference methods, hence are recommended for quality control and routine