RP—HPLC法测定不同产地枳实中的柚皮苷、橙皮苷及新橙皮苷

A method was established to determine the contents of Naringin, Hesperidin and Neogesperidin in Frutus aurantii immaturus. Reversed-phase HPLC was used to find the contents of Naringin, Hesperidin and Neogesperidin in Frutus aurantii immaturus. The chromatographic conditions were as follows: Hyspersil ODS2(250 mm×4.6 mm, 5 μm elite, Dalian), mobile phase of acetonitrile-water (20:80), flow rate: 1.0 mL/min, detection wavelength: 283 nm and the column temperature: 30℃. The calibration curves were linear in the range of 1.00~7.50 μg for Naringin and Neohesperidin, and 0.403.00 μg for Hesperidin. The average recovery of Naringin Hesperidin and Neogesperidin were 100.56% (RSD=0.55%), 100.20% (RSD=1.00%), 101.14% (RSD=1.74%) respectively. This method is simple, accurate, reliable and has good reproducibility. It can be used for the quality control for the production of medicine containing Frutus aurantii immaturus.