Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shaan 253

Eight γ-gliadin genes (GenBank accession number GQ87170 to GQ87177) were cloned from wheat (Triticum aestivum L.) cultivar Shaan 253 using three sets of specific primers, which were designed according to the known γ-gliadin gene family. All of the eight genes possess the typical structure of γ-gliadin subunits and many sequences of insent/indel in the repeat region. The deduced amino acid sequences showed that GQ871771 is a pseudogene, and nine cysteine residues existed in GQ871770, GQ871772, GQ871776, and GQ87177. Promoter sequences analysis indicated that there were six SNPs in endosperm box of GQ871770, GQ871772, GQ87174, and GQ871776, and two of them appeared in the GCN4-like motif. A model for endosperm box of storage protein with better representation was constructed using the online tool WebLogo3. Phylogenetic analysis also confirmed that the cloned sequences belong to γ-gliadin gene family.