Blocking method for activated quantum dots

The invention provides a blocking method for activated quantum dots. The activated quantum dots are blocked by adopting polymer inert protein formed by cross-linking reaction of 1-ethyl-3-3-(3-dimethyl aminopropyl)-carbodiimide (EDC) and inert protein. The method disclosed by the invention has the advantages that effective blocking of the surfaces of the activated quantum dots can be ensured and protein linking in the blocking process is firmer, and thereby the sensitivity and the stability of a reagent are improved and basic performance of the quantum dot immunochromatography reagent is improved. The invention also aims at providing application of the method in an immunofluorescence assay kit for the quantum dots.