712. High Capacity Screen To Select Optimal Pre-trans-splicing Molecules for Trans-splicing Applications|[ast]|

Spliceosome Mediated RNA Trans-splicing (SMaRT™) is a platform technology to reprogram genes at the pre-mRNA level. Applications include RNA therapy and real-time molecular imaging, with each application dependent upon the nature of the sequences encoded by the pre-trans-splicing molecule (PTM). SMaRT utilizes native spliceosomes to carry out trans-splicing reactions between two RNA molecules: a pre-mRNA target present in the cell and a delivered PTM.