Bends Which Melt at 37°C Plasmid Contains Multiple Intrinsic DNA The Yersinia enterocolitica pYV Virulence

, thepositive activator of virulence genes. These bends are detectable at 30°C but melt at 37°C, the temperature atwhich the cells undergo phenotypic switching. We also show that pACYC184, a plasmid used as a reporter oftemperature-induced changes in DNA supercoiling, has a single region of intrinsic bending detected by ourassay. Topoisomers of pACYC184, with and without this bend, isolated from