FOXO1 targeting by capsaicin reduces tissue damage after testicular torsion
2018
Testicular torsion-related oxidative stress causes a sequential chain of DNA damage,
lipid peroxidation and cell death that leads to the derangement in the sperm functions
and infertility. Capsaicin that has been applied for pain relief and cancer prevention
possesses antioxidant properties which can be exploited to confer cell survival under
ischaemic testis damage. Wistar male rats weighing 150–200 g were randomly divided
into four groups: (i) sham group (all procedures except torsion of testis), (ii) ischaemia
group (TT group), (iii) three TT groups treated with different dose of capsaicin (TT +
different doses of Cap) and (iv) three control groups treated with different doses of
capsaicin (100, 500 and 1000 ug/ml). Capsaicin administration significantly decreased
the expression of pro-apoptotic factors and increased the expression of anti-apoptotic
factors. Likewise, the expression of FOXO1 is significantly increased by higher doses
of the capsaicin. Histological assessment by H&E and TUNEL method also exhibited
an improved testicular morphology and decreased apoptosis in testes. These results
suggested clinical potential for capsaicin in treatment of testicular torsion by targeting
FOXO1 and apoptotic pathways.
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