Effects of Liquor on Blood Glucose,Ins Expression and Glu Expression in Pancreatic Islet of Rats

Objective: To explore the effects of liquor( 38% vol) on fasting blood glucose,Ins expression in pancreatic islet B cells and Glu expression in pancreatic islet A cells and to offer certain theoretical basis for studying healthy and scientific drinking. Methods: Forty normal male adult SD rats were randomly divided into normal control group and experimental group. Experiment group( 30rats) were divided into 4 w group and 12 w group. The rats in different week group were further divided into low dose group( L group),middle dose group( M group) and high dose group( H group).There were 5 normal control rats in 4 w group and 12 w group respectively. The rats in experiment group received intragastric administration of liquor twice a day,one at 11 ∶ 00 am and the other 5 ∶ 00 pm,while the normal control group remain no special treatment. At the end of 4 weeks and 12 weeks,fasting blood glucose was detected. Pituitary glands were excised at the end of 4 weeks,8 weeks and12 weeks. Immunohistochemical SABC,image analysis and morphometry methods were adopted to count Ins positive cells and Glu positive cells,mean optical density and NA. Result: There was no significant differences in fasting blood glucose level between experiment group and normal control group( P 0. 05). Immunohistochemistry result showed that under the light microscope,the immunoreactive products of Glu positive cells and Ins positive cells were all brown particles in the cytoplasm. Morphological measurement results showed that there was no significant differences in the mean density and NA of Ins positive cells and Glu positive cells between experiment group and normal control group( P 0. 05). Conclusion: The administration by gavage of this brand liquor( 38% vol) with the dosage and the time period in this experiment has no significant effect on fasting blood glucose,Ins expression in pancreatic islet B cells and Glu expression in pancreatic islet A cells of rats.