A study on anti-hepatocellular carcinoma of tumor-infiltrating lymphocyte cytotoxicity activated by dendritic cells loaded with hepatocellular carcinoma antigens

目的 为探讨肝癌病人肝癌细胞抗原负载树突状细胞激活肝癌浸润淋巴细胞(TIL)的抗肿瘤作用.方法以细胞计数、间接荧光表型测定分析肝癌病人DC功能状态;MTT法测定肝癌病人DC介导TIL对肝癌细胞的杀伤活性.结果肝癌病人DC表达CD1a、CD80、CD83和HLA-DR分子水平,DC介导的TIL对肝癌细胞的杀伤活性明显低于健康对照组(P<0.05,0.01).结论肝癌病人存在DC功能缺陷,致使其介导的TIL对肝癌细胞的杀伤作用明显降低。