Light control method for dendrobium officinale tissue culture

2009 
The invention relates to a light control method for dendrobium officinale tissue culture, which comprises the following steps of: obtaining powdery seeds after dendrobium officinale fruits are sterilized, culturing the powdery seeds through a seed germination culture medium, and germinating the cultured seeds to form protocorms. The method is characterized in that: in the tissue culture process of dendrobium officinale seedlings, a light emitting diode (LED) plant growth illumination system is used as a light source, and different illumination treatment is implemented according to different stages of dendrobium officinale tissue culture. The different illumination treatment is implemented according to different growth stages so that the dendrobium officinale seedlings can furthest utilize light energy for photosynthesis and the highest growth amount is obtained. Experimental results show that: the culture period of the dendrobium officinale seedlings can be effectively shortened according to the technical scheme, the time from the seeds to the rooted finished seedlings is only 150 to 180 days, the growth period is shortened by 30 to 60 days compared with the culture under a common fluorescent lamp, and the seedlings cultured by the method have stable hereditary character and good quality.
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